Human Whole Blood: DNA & RNA Extraction Protocols & Kit Selection Guide

DNA extraction from whole blood isolates stable genomic DNA from leukocytes, suitable for long-term storage and applications like NGS and PCR. RNA extraction captures messenger RNA reflecting active gene expression at the time of collection, requiring RNase-free conditions and faster processing. Different buffers, protocols, and purity requirements apply to each nucleic acid type.

The Cambrian Whole Blood gDNA Kit requires a minimum of 200 µL of whole blood (scalable up to 2 mL). The Cambrian Blood RNA Isolation Kit accepts 500 µL to 2 mL of whole blood, with RNA yields scaling linearly from approximately 2.17 µg at 0.5 mL input to 9.61 µg at 2 mL input.

Extracted gDNA is validated for PCR-based assays, Next-Generation Sequencing (NGS), microarray analysis, genetic testing, and clinical diagnostics. Isolated RNA with RIN values of 8.6–9.7 supports RNA-Seq, transcriptome analysis, gene expression profiling, BCR-ABL testing, epigenetic studies, and high-throughput molecular diagnostic workflows.

The Cambrian Whole Blood gDNA Kit completes on-deck processing in just 28 minutes, including a 10-minute lysis step. The Cambrian Blood RNA Isolation Kit requires a 50-minute on-deck time with a 40-minute lysis step. Both kits run fully automated on the Manta platform, minimizing hands-on labor.

Extracted gDNA purity is assessed using A260/280 ratios (target: 1.8–2.0) and A260/230 ratios (target: 2.0–2.2), indicating protein and solvent contamination levels respectively. RNA quality is measured by RNA Integrity Number (RIN), validated at 8.6–9.7 on the Agilent TapeStation System, confirming suitability for sensitive downstream applications.

Yes. Both kits are designed exclusively for the Manta automated nucleic acid extraction platform by Cambrian Bioworks. Pre-filled, non-reusable cartridges are loaded onto the Manta instrument, which handles lysis, binding, washing, and elution automatically. The system supports 1 to 32 samples per run, accommodating variable throughput without batching pressure.