Digital PCR Kits for cfDNA Quantification & ctDNA Detection

Digital PCR system costs vary widely by platform, throughput, and vendor, typically ranging from $30,000 to over $100,000 USD for instruments. Consumable kits like the OncoSeek cfDNA Extraction Kit are priced separately and available in 50-reaction packs. Contact Cambrian Bioworks directly via cambrianbioworks.com or connect@cambrianbioworks.com for specific pricing and tester kit availability.

Traditional PCR amplifies DNA and measures fluorescence relative to a standard curve, providing relative quantification. Digital PCR partitions the reaction into thousands of individual droplets or chambers, enabling absolute quantification without standards. Digital PCR is significantly more sensitive for detecting rare variants such as ctDNA mutations at low allelic frequencies, making it the preferred method for oncology liquid biopsy workflows.

The OncoSeek cfDNA Extraction Kit uses bead-based purification optimized for plasma samples, requiring only 1 mL of plasma — half the volume required by many competitors — while delivering 5–20× higher cfDNA yield. This is especially advantageous for pediatric oncology, limited-volume clinical samples, and longitudinal monitoring studies where preserving sample volume is critical.

The extracted cfDNA is validated for use in Next-Generation Sequencing (NGS) library preparation, quantitative PCR (qPCR), and digital PCR including droplet digital PCR (ddPCR) workflows. The kit preferentially captures 150–200 bp tumor-derived cfDNA fragments, enriching ctDNA fractions for improved sensitivity in mutation profiling and minimal residual disease detection.

Yes. The OncoSeek cfDNA Extraction Kit uses bead-based purification that is fully compatible with automation platforms, including the Beluga automated liquid handler from Cambrian Bioworks. Automation reduces manual effort, minimizes variability, and enables consistent high-throughput cfDNA extraction in approximately 55 minutes per batch.

Cambrian Bioworks' kits are manufactured under ISO 13485 quality management standards and carry the CE-IVD mark, ensuring regulatory-grade reliability. The company also holds CDSCO IVD certification for its manufacturing operations. These certifications make the kits a trusted choice for oncology research and diagnostic labs across Asia and Europe.

Cambrian Bioworks' digital PCR kits are designed for oncology research labs, clinical diagnostics centers, genomics and sequencing labs, and biobanking and research institutions. Applications span ctDNA detection, MRD monitoring, liquid biopsy assay development, NGS library preparation, and longitudinal plasma sample collection workflows. The kits are trusted by 67+ labs globally, including Tata Memorial Hospital and ACTREC.