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CBWM068.64 | Cambrian Whole Blood gDNA Isolation Kit

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The Manta's Whole Blood Isolation Kit is built for efficient, high-quality DNA extraction from whole blood and similar sample types. It accommodates volumes from 200 µL to 2 mL. In under 30 minutes, you'll achieve high molecular weight DNA, ready for applications like PCR, NGS, genetic testing and more. Powered by advanced magnetic bead technology, the kit ensures consistent results, enabling

advancements in your research or clinical diagnostics.

The Manta's Whole Blood Isolation Kit is built for efficient, high-quality DNA extraction from whole blood and similar sample types. It accommodates volumes from 200 µL to 2 mL. In under 30 minutes, you'll achieve high molecular weight DNA, ready for applications like PCR, NGS, genetic testing and more. Powered by advanced magnetic bead technology, the kit ensures consistent results, enabling

advancements in your research or clinical diagnostics.

Available kit size

64 reactions

Designed with efficiency

Designed

with efficiency

Designed

with efficiency

Run single samples

Automation shouldn’t tie you down to batch sizes. With our cartridge-based system, you can process even a single blood sample without wasting additional buffers or plastics.

Automation shouldn’t tie you down to batch sizes. With our cartridge-based system, you can process even a single blood sample without wasting additional buffers or plastics.

Fast and effortless workflows

The whole blood kit processes samples in just 27 minutes. Peel the seal, add your lysate, and let Manta handle the rest.

The whole blood kit processes samples in just 27 minutes. Peel the seal, add your lysate, and let Manta handle the rest.

Run distinct samples

Manta processes different sample types simultaneously without compromising performance, ensuring seamless flexibility for diverse workflows.

Manta processes different sample types simultaneously without compromising performance, ensuring seamless flexibility for diverse workflows.

Adaptable throughput

Process just 1 to 32 samples in a single run.

Process just 1 to 32 samples in a single run.

Flexible input and elution volumes

We understand that every lab has its nuances which is why we designed the kit to be adjustable with input and elution volumes to optimize for concentration or yield.

We understand that every lab has its nuances which is why we designed the kit to be adjustable with input and elution volumes to optimize for concentration or yield.

Versatile applications

The extracted DNA is of high quality and is suitable for a wide range of downstream applications, including NGS, PCR, microarrays, and more.

The extracted DNA is of high quality and is suitable for a wide range of downstream applications, including NGS, PCR, microarrays, and more.

Trusted by

experts in the lab

Banaras Hindu University, Varanasi

Prof. Gyaneshwar Chawbe’s lab is using Manta's Whole Blood Kit to build a DNA biobank to study how endogamy impacts genetic disorders and drives advancements in personalized treatments.

Institute of Human Genetics, Ahmedabad

Dr. Harsh Sheth’s lab uses Manta's Whole Blood Kit to support diagnostic services, counseling, and translational research.

Strand Lifesciences, Bengaluru

Dr. Arun Hariharan's lab uses Manta's Whole Blood Kit for disease diagnostics, variant interpretation, and reporting.

The Advanced Centre for

Treatment, Research and Education in Cancer (ACTREC), Mumbai

Dr. Nikhil Patkar's lab uses Manta's Whole Blood Kit for the diagnosis, prognosis, and monitoring of patients with blood cancers.

Trusted by

experts in the lab

Banaras Hindu University, Varanasi

Prof. Gyaneshwar Chawbe’s lab is using Manta's Whole Blood Kit to build a DNA biobank to study how endogamy impacts genetic disorders and drives advancements in personalized treatments.

Institute of Human Genetics, Ahmedabad

Dr. Harsh Sheth’s lab uses Manta's Whole Blood Kit to support diagnostic services, counseling, and translational research.

Strand Lifesciences, Bengaluru

Dr. Arun Hariharan's lab uses Manta's Whole Blood Kit for disease diagnostics, variant interpretation, and reporting.

The Advanced Centre for

Treatment, Research and Education in Cancer (ACTREC), Mumbai

Dr. Nikhil Patkar's lab uses Manta's Whole Blood Kit for the diagnosis, prognosis, and monitoring of patients with blood cancers.

Trusted by

experts in the lab

Banaras Hindu University, Varanasi

Prof. Gyaneshwar Chawbe’s lab is using Manta's Whole Blood Kit to build a DNA biobank to study how endogamy impacts genetic disorders and drives advancements in personalized treatments.

Institute of Human Genetics, Ahmedabad

Dr. Harsh Sheth’s lab uses Manta's Whole Blood Kit to support diagnostic services, counseling, and translational research.

Strand Lifesciences, Bengaluru

Dr. Arun Hariharan's lab uses Manta's Whole Blood Kit for disease diagnostics, variant interpretation, and reporting.

The Advanced Centre for

Treatment, Research & Education

in Cancer (ACTREC), Mumbai

Dr. Nikhil Patkar's lab uses Manta's Whole Blood Kit for the diagnosis, prognosis, and monitoring of patients with blood cancers.

This kit is compatible with:

This kit is compatible with

Whole blood

Whole blood

Whole blood

Whole blood

Whole blood

Whole blood

Cell cultures

Cell cultures

Cell cultures

Cell cultures

Cell cultures

Cell cultures

Bacterial cells

Bacterial cells

Bacterial cells

Bacterial cells

Bacterial cells

Bacterial cells

Explore our full range

of kits for other

sample types.

Explore our full range

of kits for other

sample types.

Technical specifications

Attributes

Description

Compatable sample types

Whole blood, cell cultures, bacterial cells

Method of purification

Magnetic bead-based technology

Cartridges

Pre-filled, 1 cartridge for 1 sample, non-reusable

Lysis buffers

Included in the box

On-deck time

28 Mins

Lysis time

10 Mins

Input volume

200 µL (can be modified)

Lysis time

100 µL (can be modified)

Pack size

64 reactions

Kit content (64 reactions)

Cartridges x64, Blood lysis buffer, Proteinase K (lyophilized),

Proteinase K Diluent, Plastic combs x10, IFU

Storage conditions

Cartridges are to be stored at room temperature, pK is to be stored at 4C before reconstitution and at -20C after reconstitution

Technical specifications

Attributes

Description

Compatable sample types

Whole blood, cell cultures, bacterial cells

Method of purification

Magnetic bead-based technology

Cartridges

Pre-filled, 1 cartridge for 1 sample, non-reusable

Lysis buffers

Included in the box

On-deck time

28 Mins

Lysis time

10 Mins

Input volume

200 µL (can be modified)

Lysis time

100 µL (can be modified)

Pack size

64 reactions

Kit content (64 reactions)

Cartridges x64, Blood lysis buffer, Proteinase K (lyophilized),

Proteinase K Diluent, Plastic combs x10, IFU

Storage conditions

Cartridges are to be stored at room temperature, pK is to be stored at 4C before reconstitution and at -20C after reconstitution

Technical specifications

Attributes

Description

Compatable sample types

Whole blood, cell cultures, bacterial cells

Method of purification

Magnetic bead-based technology

Cartridges

Pre-filled, 1 cartridge for 1 sample, non-reusable

Lysis buffers

Included in the box

On-deck time

28 Mins

Lysis time

10 Mins

Input volume

200 µL (can be modified)

Lysis time

100 µL (can be modified)

Pack size

64 reactions

Kit content (64 reactions)

Cartridges x64, Blood lysis buffer, Proteinase K (lyophilized),

Proteinase K Diluent, Plastic combs x10, IFU

Storage conditions

Cartridges are to be stored at room temperature, pK is to be stored at 4C before reconstitution and at -20C after reconstitution

Technical specifications

Attributes

Description

Compatable sample types

Whole blood, cell cultures, bacterial cells

Method of purification

Magnetic bead-based technology

Cartridges

Pre-filled, 1 cartridge for 1 sample, non-reusable

Lysis buffers

Included in the box

On-deck time

28 Mins

Lysis time

10 Mins

Input volume

200 µL (can be modified)

Lysis time

100 µL (can be modified)

Pack size

64 reactions

Kit content (64 reactions)

Cartridges x64, Blood lysis buffer, Proteinase K (lyophilized),

Proteinase K Diluent, Plastic combs x10, IFU

Storage conditions

Cartridges are to be stored at room temperature, pK is to be stored at 4C before reconstitution and at -20C after reconstitution

Performance metrics

Sample condition : Properly collected and stored blood at 4°C

Sample input volume : 200 µL

Elution volume : 100 µL

Yield (ng/uL)

A260/280

A260/230

6 - 15 ng

1.8 - 2.0

2.0 - 2.2

Performance metrics

Sample condition : Properly collected and stored blood at 4°C

Sample input volume : 200 µL

Elution volume : 100 µL

Yield (ng/uL)

A260/280

A260/230

6 - 15 ng

1.8 - 2.0

2.0 - 2.2

Performance metrics

Sample condition : Properly collected and stored blood at 4°C

Sample input volume : 200 µL

Elution volume : 100 µL

Yield (ng/uL)

A260/280

A260/230

6 - 15 ng

1.8 - 2.0

2.0 - 2.2

Performance metrics

Sample condition : Properly collected and stored blood at 4°C

Sample input volume : 200 µL

Elution volume : 100 µL

Yield (ng/uL)

A260/280

A260/230

6 - 15 ng

1.8 - 2.0

2.0 - 2.2

Toolkit

IFU for Cambrian Whole Blood

gDNA Isolation Kit I CBWM068.64

600 µL of blood as the starting sample volume with Manta

Material safety data sheet

User manual for Manta

Toolkit

IFU for Cambrian Whole Blood

gDNA Isolation Kit I CBWM068.64

600 µL of blood as the starting sample volume with Manta

Material safety data sheet

User manual for Manta

Toolkit

IFU for Cambrian Whole Blood

gDNA Isolation Kit I CBWM068.64

600 µL of blood as the starting sample volume with Manta

Material safety data sheet

User manual for Manta

Toolkit

IFU for Cambrian Whole Blood

gDNA Isolation Kit I CBWM068.64

600 µL of blood as the starting sample volume with Manta

Material safety data sheet

User manual for Manta

FAQs

How long does it take to extract DNA from whole blood samples?

The entire workflow takes about 37 minutes with 10 minutes of lysis outside of the machine and 27 minutes on deck.

Does the lysis happen outside of the Manta?

Yes, lysis occurs outside the machine. The resulting lysate is then added to the cartridge, which is placed on the device for further processing.

Do you supply lysis buffers?

Yes, we supply all the required buffers and consumables for the extraction workflow.

How does hemoglobin contamination affect blood lysis, and how can it be minimized?

Hemoglobin can hinder cell lysis and affect downstream DNA purity. Using Buffer BL for effective RBC lysis ensures a clean separation of genomic DNA.

Can old or stored blood samples cause lysis difficulties?

Yes, aged blood samples may experience hemolysis and degradation, leading to reduced DNA yield. To minimize this, process blood samples within 24 hours or store them at -80°C for long-term preservation.

What can I do if there are beads in the elute?

If there are beads in the elute, collect the elute in a microcentrifuge tube and centrifuge at 14000 rpm for 1 minute, and transfer the cleared elute to a fresh microcentrifuge tube.

Can I use clotted blood for DNA extraction?

Do not use clotted blood for genomic DNA extraction. In the case of microclots, ensure that the samples are lysed efficiently at 70°C for 15 minutes, with thorough vortexing.

What is the expected DNA yield and purity from blood samples?

The yield ranges from 5–12 μg per 200 μL of blood with A260/A280 purity ratios between 1.8 and 2.0​.

FAQs

How long does it take to extract DNA from whole blood samples?

The entire workflow takes about 37 minutes with 10 minutes of lysis outside of the machine and 27 minutes on deck.

Does the lysis happen outside of the Manta?

Yes, lysis occurs outside the machine. The resulting lysate is then added to the cartridge, which is placed on the device for further processing.

Do you supply lysis buffers?

Yes, we supply all the required buffers and consumables for the extraction workflow.

How does hemoglobin contamination affect blood lysis, and how can it be minimized?

Hemoglobin can hinder cell lysis and affect downstream DNA purity. Using Buffer BL for effective RBC lysis ensures a clean separation of genomic DNA.

Can old or stored blood samples cause lysis difficulties?

Yes, aged blood samples may experience hemolysis and degradation, leading to reduced DNA yield. To minimize this, process blood samples within 24 hours or store them at -80°C for long-term preservation.

What can I do if there are beads in the elute?

If there are beads in the elute, collect the elute in a microcentrifuge tube and centrifuge at 14000 rpm for 1 minute, and transfer the cleared elute to a fresh microcentrifuge tube.

Can I use clotted blood for DNA extraction?

Do not use clotted blood for genomic DNA extraction. In the case of microclots, ensure that the samples are lysed efficiently at 70°C for 15 minutes, with thorough vortexing.

What is the expected DNA yield and purity from blood samples?

The yield ranges from 5–12 μg per 200 μL of blood with A260/A280 purity ratios between 1.8 and 2.0​.

FAQs

How long does it take to extract DNA from whole blood samples?

Does the lysis happen outside of the Manta?

Do you supply lysis buffers?

How does hemoglobin contamination affect blood lysis, and how can it be minimized?

Can old or stored blood samples cause lysis difficulties?

What can I do if there are beads in the elute?

Can I use clotted blood for DNA extraction?

What is the expected DNA yield and purity from blood samples?

FAQs

How long does it take to extract DNA from whole blood samples?

The entire workflow takes about 37 minutes with 10 minutes of lysis outside of the machine and 27 minutes on deck.

Does the lysis happen outside of the Manta?

Yes, lysis occurs outside the machine. The resulting lysate is then added to the cartridge, which is placed on the device for further processing.

Do you supply lysis buffers?

Yes, we supply all the required buffers and consumables for the extraction workflow.

How does hemoglobin contamination affect blood lysis, and how can it be minimized?

Hemoglobin can hinder cell lysis and affect downstream DNA purity. Using Buffer BL for effective RBC lysis ensures a clean separation of genomic DNA.

Can old or stored blood samples cause lysis difficulties?

Yes, aged blood samples may experience hemolysis and degradation, leading to reduced DNA yield. To minimize this, process blood samples within 24 hours or store them at -80°C for long-term preservation.

What can I do if there are beads in the elute?

If there are beads in the elute, collect the elute in a microcentrifuge tube and centrifuge at 14000 rpm for 1 minute, and transfer the cleared elute to a fresh microcentrifuge tube.

Can I use clotted blood for DNA extraction?

Do not use clotted blood for genomic DNA extraction. In the case of microclots, ensure that the samples are lysed efficiently at 70°C for 15 minutes, with thorough vortexing.

What is the expected DNA yield and purity from blood samples?

The yield ranges from 5–12 μg per 200 μL of blood with A260/A280 purity ratios between 1.8 and 2.0​.

Try Manta for

whole blood samples

Try Manta for

whole blood samples

Try Manta for

whole blood samples

Schedule a demo with us and see how Manta can make your workflows more efficient.

Schedule a demo with us and see how Manta can make your workflows more efficient.

Schedule a demo with us and see how Manta can make your workflows more efficient.

Schedule your demo now

Schedule your demo now

Schedule your demo now

Schedule your demo now